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KMID : 0365819700100010167
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Journal of Pusan Medical College
1970 Volume.10 No. 1 p.167 ~ p.175
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Serological Studies on the Experimental Serum Sickness
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梁學道/Yang, Hak Do
黃仁壽/金政禧/Hwang, In Soo/Kim, Chung Hi
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Abstract
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White rabbits weighing approximately 2 to 2.5kg were used throughout this study. Each experimental animal received a single intravenous injection of horse serum. After intravenous injection of horse serum, samples of blood were obtained from the marginal ear veins at varying intervals, and the sera were tested for the presence of antigen(horse serum) or antibody by the precipitation techniques. Sera were also subjected for hemagglutination and hemagglutination inhibition tests. The results obtained are summarized as follows:
1. Antigen remained in the sera of all the rabbits for as long- as 10 days after injection of horse serum. By the 13th day, of all the aminals had eliminated the horse serum and produced considerable high level of antibody.
2. Formalinized-tanned-cell hemagglutination test can be applied for the demonstration of horse serum antigen and its antibody with extremely sensitive results than the conventional precipitation techniques.
3. The linear relationship (optimal proportions) that exits as shown in the Table 4, a 2-fold dilution of antibody required approximately one-half the amount of antigen (horse serum) to inhibit the hemagglutination test.
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